Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress

Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez

and Txnip mRNA were examined over a 24 hour period. Txnip mRNA was induced rapidly, with maximum expression seen at 1.5 hours, followed by a marked decrease coincident with the increased expression of Gm15441 ( Figure 2E ). This time course is markedly different from that of 14 other WY-14643-inducible protein coding and lncRNA genes examined, where peak induction occurred 6 to 12 hours after WY-14643 treatment (Figure S1 ), as was also found for Gm15441. The unusual kinetics seen with Txnip -early induction followed by repression coinciding with the activation of Gm15441 -indicate that Txnip is a PPARA target gene whose expression in liver is inversely regulated with that of Gm15441 after PPARA activation.