Author: João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad
Title: A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells Document date: 2018_12_26
ID: 1kugu5zk_14
Snippet: In designing genetic constructs, an important consideration is the relative doses of expression strengths between respective genes. Within the MTK, we have seventeen characterized constitutive promoters that span a spectrum of transcriptional expression strengths. We include promoters from a mix of human, mouse and viral origin for a range of expression, alternatives to swap in the event of silencing effects, and a balance of native and transgene.....
Document: In designing genetic constructs, an important consideration is the relative doses of expression strengths between respective genes. Within the MTK, we have seventeen characterized constitutive promoters that span a spectrum of transcriptional expression strengths. We include promoters from a mix of human, mouse and viral origin for a range of expression, alternatives to swap in the event of silencing effects, and a balance of native and transgene promoters for conventional cell lines.
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