Selected article for: "landing pad system and site specific integration"
Author: João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad
Title: A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells
  • Document date: 2018_12_26
    • ID: 1kugu5zk_22
    Snippet: When delivering synthetic genetic circuits, it can be essential to have site-specific, single copy integration. In addition, it is important that this integration is efficient and reliable. In order to accomplish this functionality, we included in the MTK a BxBI-dependent landing pad system for integrating synthetic circuits in a locus of choice. The landing pad system is divided into two parts: first, a landing pad cell line; and second, a landi.....
    Document: When delivering synthetic genetic circuits, it can be essential to have site-specific, single copy integration. In addition, it is important that this integration is efficient and reliable. In order to accomplish this functionality, we included in the MTK a BxBI-dependent landing pad system for integrating synthetic circuits in a locus of choice. The landing pad system is divided into two parts: first, a landing pad cell line; and second, a landing pad transfer vector. As a proof of concept we built a HEK293T cell line with a BxBI landing site in the hAAVS1 locus (hAAVS1 LP), identified by mRuby2 expression and Hygromycin resistance, and tested its ability for site specific recombination.

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