A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells

João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad

The MTK encompasses part vector categories 1-8 that are sufficient to build and deliver a vast combinatorial library of genetic constructs to cells (Fig. 1b) . Parts 2, 3 and 4 form the core of the TU, specifying the 5' UTR, coding, and 3' UTR sequences, respectively. Part 2 corresponds to promoter sequences that can be specified to drive variable constitutive or . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/506188 doi: bioRxiv preprint inducible expression or recruit diverse host polymerases, such as polymerase III for non-coding RNA transcription. Part 3 vectors are canonical coding sequences that are typically proteins of interest. Part 4 refers to 3' UTR sequences that encode polyadenylation (pA) sequences that terminate transcription, or spacer sequences that couple transcription to the downstream TU.