Author: Xiankun Zeng; Jun Liu; April Babka; Brian Kearney; Sheli Radoshitzky; Jens Kuhn
Title: Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens Document date: 2020_4_22
ID: nzwe7hpg_28
Snippet: The FFPE specimen-compatible rabbit and mouse anti-SARS-CoV-2 antibodies we 145 characterized here can be used to map the cellular targets of SARS-CoV-2 in various organs 146 using multiplex IFA in addition to detecting viral infection. RNAscope ISH is a relatively novel 147 ISH platform with high-sensitivity and low-background due to its unique "ZZ" probe design 148 (33). This platform has been widely used to detect viruses both in human and ani.....
Document: The FFPE specimen-compatible rabbit and mouse anti-SARS-CoV-2 antibodies we 145 characterized here can be used to map the cellular targets of SARS-CoV-2 in various organs 146 using multiplex IFA in addition to detecting viral infection. RNAscope ISH is a relatively novel 147 ISH platform with high-sensitivity and low-background due to its unique "ZZ" probe design 148 (33). This platform has been widely used to detect viruses both in human and animal tissues (27, 149 author/funder. All rights reserved. No reuse allowed without permission. can be applied to detect viral RNA in both human and animal tissue samples. The dual staining 154 we developed to detect SARS-CoV-2 viral antigen and RNA in the same FFPE section can more 155 precisely detect SARS-CoV-2 because a positive IHC or ISH signal alone may originate from 156 remaining free viral antigen or degenerating RNA fragments rather than from viral particles.
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