Selected article for: "direct analysis and human saliva"
Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus
  • Document date: 2018_9_23
    • ID: n5liudlg_26
    Snippet: Saliva is a convenient, non-invasive clinical sample that is increasingly being used for first-line rapid detection of pathogens including ZIKV. 40, 41 To demonstrate the utility of our smart molecular diagnostic system in rapid salivary diagnostics, surrogate clinical samples were prepared by spiking human saliva with different amounts of Asian lineage ZIKV virions (Supplementary Figure 1 ) followed by direct analysis using singleplex degenerate.....
    Document: Saliva is a convenient, non-invasive clinical sample that is increasingly being used for first-line rapid detection of pathogens including ZIKV. 40, 41 To demonstrate the utility of our smart molecular diagnostic system in rapid salivary diagnostics, surrogate clinical samples were prepared by spiking human saliva with different amounts of Asian lineage ZIKV virions (Supplementary Figure 1 ) followed by direct analysis using singleplex degenerate NS1 LAMP-OSD and degenerate 4-Plex-LAMP-2GO assays. To verify diagnostic accuracy, saliva samples spiked with an unrelated RNA virus were also tested. To ascertain diagnostic speed, assay fluorescence was imaged using a cellphone at various intervals.

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