Development of a dual-labeled, hydrolysis probe-based, real-time quantitative PCR assay for detection of both genotypes of duck circovirus-1 (DuCV-1) and DuCV-2

Zhang, Lin; Jiang, Wenming; Zhang, Fuyou; Li, Yang; Li, Jinping; Liang, Shaobo; Yu, Xiaohui; Peng, Cheng; Liu, Shuo; Wang, Jingjing; Sun, Shuhong; Liu, Hualei

In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using other duck pathogens. The detection limit was 20 copies per µL. The intra-assay coefficients of variation (CVs) were ≤ 0.73% and the inter-assay CVs were ≤ 1.89%. No cross-reaction occurred with other duck pathogens. In addition, the qPCR assay was successfully applied to the simultaneous detection of DuCV-1 and DuCV-2 in clinical field samples. Therefore, this assay will be useful for laboratory diagnosis and epidemiological field studies of DuCV. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11262-021-01862-9.