More than efficacy revealed by single-cell analysis of antiviral therapeutics

Wu Liu; Mehmet U. Caglar; Zhangming Mao; Andrew Woodman; Jamie J. Arnold; Claus O. Wilke; Craig E. Cameron

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. to a density of 5 × 10 5 cells/mL, and load the microfluidic device. Each well is monitored for 109 eGFP fluorescence every 30 min for a 24 h period, which gives rise to a population of time 110 courses exhibiting substantial between-cell variability (Fig. S4a) . We plot the percentage of 111 infected cells as a function of drug concentration and fit to a hyperbola to obtain a value for the 112 IC50 value. In the case of 2'-C-Me-A, concentrations greater than 50 µM exhibit toxicity 113 (Stuyver et al., 2006) ; therefore, these data do not fit to a hyperbola (Fig. S4b) . It is clear from 114 this experiment, however, that the approach is sensitive enough to acquire data for a complete 115 dose-response analysis (Fig S4b) . 116 All rights reserved. No reuse allowed without permission.