Author: João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad
Title: A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells Document date: 2018_12_26
ID: 1kugu5zk_2
Snippet: Conventional approaches to produce DNA vectors involve the PCR amplification of DNA fragments from different sources, which are later ligated following digestion with restriction enzymes. This procedure is time consuming, laborious, prone to errors and requires many control steps when subcloning (e.g. sequencing). Due to the shrinking cost of commercial DNA synthesis, ordering gene fragments or entire genes provides a potential avenue to reliably.....
Document: Conventional approaches to produce DNA vectors involve the PCR amplification of DNA fragments from different sources, which are later ligated following digestion with restriction enzymes. This procedure is time consuming, laborious, prone to errors and requires many control steps when subcloning (e.g. sequencing). Due to the shrinking cost of commercial DNA synthesis, ordering gene fragments or entire genes provides a potential avenue to reliably build libraries of DNA vectors. While this technology increases the diversity of parts that can be cloned and removes a DNA source requirement, current pricing, size limitation, and turnaround time remain a significant bottleneck.
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