Author: João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad
Title: A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells Document date: 2018_12_26
ID: 1kugu5zk_57
Snippet: For the analysis of promoter, 3'UTR expression and hAAVS1 LP expression, cells were collected in 96 well plates (Corning) and measured using an LSR2 flow cytometer (BD). mAzamiGreen (excitation at 488 nm, emission at 530 nm), mRuby2 or mScarlet (excitation at 561 nm, emission between 610 and 620 nm) and tagBFP (excitation at 355 nm, emission at 450 nm) fluorescence levels were recorded for 10000 events. Gating of single cells, normalization of fl.....
Document: For the analysis of promoter, 3'UTR expression and hAAVS1 LP expression, cells were collected in 96 well plates (Corning) and measured using an LSR2 flow cytometer (BD). mAzamiGreen (excitation at 488 nm, emission at 530 nm), mRuby2 or mScarlet (excitation at 561 nm, emission between 610 and 620 nm) and tagBFP (excitation at 355 nm, emission at 450 nm) fluorescence levels were recorded for 10000 events. Gating of single cells, normalization of fluorescence levels, and statistical analysis was performed with custom python scripts. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/506188 doi: bioRxiv preprint (excitation at 561nm, collection between 590 and 650nm) and iRFP713 (excitation at 640nm, collection between 665 and 736nm). An Andor Zyla 4.2 sCMOS was used to detect the images and pixel size was 325nm.
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