Selected article for: "Qiagen RNeasy kit and RNeasy kit"
Author: Aran Singanayagam; Joseph Footitt; Benjamin T Kasdorf; Matthias Marczynski; Michael T Cross; Lydia J Finney; Maria-Belen Trujillo Torralbo; Maria Calderazzo; Jie Zhu; Julia Aniscenko; Thomas B Clarke; Philip L Molyneaux; Nathan W Bartlett; Miriam F Moffatt; William O Cookson; Jadwiga Wedzicha; Christopher M Evans; Oliver Lieleg; Patrick Mallia; Sebastian L Johnston
Title: MUC5AC drives COPD exacerbation severity through amplification of virus-induced airway inflammation
  • Document date: 2019_7_22
    • ID: gg2ctmn7_70
    Snippet: For human studies, total RNA was extracted from sputum cells (RNeasy kit, Qiagen) and 2 µg was used for cDNA synthesis (Omniscript RT kit). For mouse models, total RNA was extracted from the right upper lobe of mouse lung and placed in RNA later, prior to RNA extraction and cDNA synthesis. Quantitative PCR (qPCR) was carried out using previously described specific primers and probes for human and mouse MUC5AC and MUC5B and rhinovirus RNA copy nu.....
    Document: For human studies, total RNA was extracted from sputum cells (RNeasy kit, Qiagen) and 2 µg was used for cDNA synthesis (Omniscript RT kit). For mouse models, total RNA was extracted from the right upper lobe of mouse lung and placed in RNA later, prior to RNA extraction and cDNA synthesis. Quantitative PCR (qPCR) was carried out using previously described specific primers and probes for human and mouse MUC5AC and MUC5B and rhinovirus RNA copy numbers 23, 47, 49 , and normalized to 18S rRNA.

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