Network-based Drug Repurposing for Human Coronavirus

Yadi Zhou; Yuan Hou; Jiayu Shen; Yin Huang; William Martin; Feixiong Cheng

is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.02.03.20020263 doi: medRxiv preprint proteins are either the direct targets of HCoV proteins or are involved in crucial pathways of HCoV infection. The HCoV-host interactome network is shown in Figure 3A . We identified several hub proteins including JUN, XPO1, NPM1, and HNRNPA1, with the highest number of connections within the 119 proteins. KEGG pathway enrichment analysis revealed multiple significant biological pathways (adjusted P value < 0.05), including measles, RNA transport, NF-kappa B signaling, Epstein-Barr virus infection, and influenza ( Figure 3B ). Gene ontology (GO) biological process enrichment analyses further confirmed multiple viral infection-related processes (adjusted P value < 0.001), including viral life cycle, modulation by virus of host morphology or physiology, viral process, positive regulation of viral life cycle, transport of virus, and virion attachment to host cell ( Figure 3C ). We then mapped the known drug-target network (see Methods) into the HCoV-host interactome to search for druggable, cellular targets.