Author: Dannielle Wellington; Zixi Yin; Liwei Zhang; Jessica Forbester; Kerry Kite; Henry Laurenson-Schafer; Shokouh Makvandi-Nejad; Boquan Jin; Emma Bowes; Krishnageetha Manoharan; David Maldonado-Perez; Clare Verill; Ian Humphreys; Tao Dong
Title: Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infection Document date: 2019_12_23
ID: ahs2wdus_14
Snippet: Embryoid Bodies (EBs). On day 5, EBs were used for generation of myeloid 199 precursor cells by plating into 6-well tissue culture treated plates (Corning) To test for reproducibility between CyTOF runs, the same donor was 243 included in each run. 100 mL of heparinized blood was drawn from a healthy 244 control donor, PBMCs were isolated and aliquots were frozen (90% fetal 245 bovine serum+10% dimethyl sulfoxide) and stored in liquid nitrogen un.....
Document: Embryoid Bodies (EBs). On day 5, EBs were used for generation of myeloid 199 precursor cells by plating into 6-well tissue culture treated plates (Corning) To test for reproducibility between CyTOF runs, the same donor was 243 included in each run. 100 mL of heparinized blood was drawn from a healthy 244 control donor, PBMCs were isolated and aliquots were frozen (90% fetal 245 bovine serum+10% dimethyl sulfoxide) and stored in liquid nitrogen until use. 246
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