Author: Katrin, Zwirglmaier; Maria, Weyh; Christian, Krüger; Rosina, Ehmann; Katharina, Müller; Roman, Wölfel; Stoecker, Kilian
Title: Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA) Cord-id: jw7x1lso Document date: 2021_1_27
ID: jw7x1lso
Snippet: In the current pandemic of SARS-CoV-2, rapid identification of infected individuals is crucial for management and control of the outbreak. However, transport of samples, sample processing and RT-qPCR analysis in laboratories are time-consuming. Here we present a prototype of a novel nucleic acid-based test format – pulse controlled amplification – that allows detection of SARS-CoV-2 directly from up to eight swab samples simultaneously without the need for RNA extraction within 25 min with a
Document: In the current pandemic of SARS-CoV-2, rapid identification of infected individuals is crucial for management and control of the outbreak. However, transport of samples, sample processing and RT-qPCR analysis in laboratories are time-consuming. Here we present a prototype of a novel nucleic acid-based test format – pulse controlled amplification – that allows detection of SARS-CoV-2 directly from up to eight swab samples simultaneously without the need for RNA extraction within 25 min with a sensitivity of 100 % for samples with a viral load of ≥ 1.6 × 10e3 copies/µl This new principle might pave the way to rapid and sensitive point of care testing.
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