Author: Xiankun Zeng; Jun Liu; April Babka; Brian Kearney; Sheli Radoshitzky; Jens Kuhn
Title: Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens Document date: 2020_4_22
ID: nzwe7hpg_26
Snippet: Detection of viral antigen by IHC and IFA has been widely used to detect infection of 140 high-consequence viruses, including SARS-CoV, EBOV, MARV, LASV, and NiV in human and 141 animal FFPE tissues (26, 28-32). Although various antigen retrieval methods can help to restore 142 the immunoreactivity of epitopes in FFPE tissues, in our experience it remains more challenging 143 to identify antibodies that binds their targets in FFPE tissues compare.....
Document: Detection of viral antigen by IHC and IFA has been widely used to detect infection of 140 high-consequence viruses, including SARS-CoV, EBOV, MARV, LASV, and NiV in human and 141 animal FFPE tissues (26, 28-32). Although various antigen retrieval methods can help to restore 142 the immunoreactivity of epitopes in FFPE tissues, in our experience it remains more challenging 143 to identify antibodies that binds their targets in FFPE tissues compared to frozen section tissues.
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