Selected article for: "long term and monitoring method"

Author: Li, Guoli; Li, Jiarui; Zhang, Henghui; Zhang, Yu; Liu, Di; Hao, Yu; Han, Junyan; Du, Juan; Zhu, Liuluan; Zeng, Yongqin; Li, Bei; Li, Rui; Song, Chuan; Zhang, Fujie; Chen, Chen; Zhao, Hongxin; Zeng, Hui
Title: Partial recovery of disturbed V-J pairing profiles of T-cell receptor in people living with HIV receiving long-term antiretroviral therapy
  • Cord-id: 39g444pq
  • Document date: 2020_6_18
  • ID: 39g444pq
    Snippet: Chronic human immunodeficiency virus (HIV) infection not only causes a gradual loss of CD4(+) T cells but also leads to a disturbance of the T cell receptor (TCR) repertoire. In people living with HIV (PLWH), monitoring TCR repertoire is challenged by the inconsistency of complementarity determining region 3 (CDR3) and limited cell numbers in clinical samples. Thus, a quantitative method is necessary for monitoring the TCR repertoire in PLWH. We characterized the TCR V-J pairing profile of naïv
    Document: Chronic human immunodeficiency virus (HIV) infection not only causes a gradual loss of CD4(+) T cells but also leads to a disturbance of the T cell receptor (TCR) repertoire. In people living with HIV (PLWH), monitoring TCR repertoire is challenged by the inconsistency of complementarity determining region 3 (CDR3) and limited cell numbers in clinical samples. Thus, a quantitative method is necessary for monitoring the TCR repertoire in PLWH. We characterized the TCR V-J pairing profile of naïve and memory CD4(+) T cells in healthy donors, HIV-infected antiretroviral therapy (ART)-naïve patients and long-term (over 5 years) ART-experienced patients by performing TCR sequencing. We developed a V-J index with 18 parameters which were subdivided into five categories (expression coverage, cumulative percentage of the top tenth percentile, diversity, intra-individual similarity and inter-individual similarity). In ART-naïve patients, 14 of the 18 parameters were significantly altered. Long-term ART recovered ten parameters. The four unrecovered parameters were related to inter-individual similarity. Therefore, these findings indicate that long-term ART could only partially recover TCR V-J pairs and introduce newly impacted V-J pairs. Moreover, these results provide new insights into the V-J pairing of the TCR and into the disturbance of TCR repertoire in HIV infection. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.1007/s11427-020-1718-2 and is accessible for authorized users.

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