Selected article for: "primer set and specific primer set"
Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus
  • Document date: 2018_9_23
    • ID: n5liudlg_48
    Snippet: The following ZIKV templates were analyzed by LAMP-OSD, LAMP-2GO, and LAMP-4GO assays: (i) zero to several thousands of copies of ZIKV synthetic RNA or genomic RNA in TE 10:0.1 buffer; (ii) ZIKV virions in TE 10:0.1 buffer and in human saliva (see sub-section "Analysis of ZIKV virions"); (iii) Un-infected and ZIKV-infected Ae. aegypti mosquitoes (see subsection "Rearing and analysis of ZIKV-infected mosquitoes"). Negative controls included (i) as.....
    Document: The following ZIKV templates were analyzed by LAMP-OSD, LAMP-2GO, and LAMP-4GO assays: (i) zero to several thousands of copies of ZIKV synthetic RNA or genomic RNA in TE 10:0.1 buffer; (ii) ZIKV virions in TE 10:0.1 buffer and in human saliva (see sub-section "Analysis of ZIKV virions"); (iii) Un-infected and ZIKV-infected Ae. aegypti mosquitoes (see subsection "Rearing and analysis of ZIKV-infected mosquitoes"). Negative controls included (i) assays without any templates; (ii) assays with non-specific templates including synthetic RNA, DENV genomic RNA, and CHIKV genomic RNA; and (iii) assays with non-specific amplicons (generated by substituting with a non-specific template and its cognate LAMP primer set).

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