Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus Document date: 2018_9_23
ID: n5liudlg_6
Snippet: Performance of these four degenerate reverse transcription LAMP-OSD assays was optimized using singleplex reactions containing different amounts of synthetic target RNA derived from Asian lineage ZIKV (Supplementary Table T3 ). All four assays could detect a few hundred copies of synthetic target RNA within 60 min without producing spurious signal (Figure 2) . Furthermore, the high signal amplitude and low noise allowed simple yes/no visual reado.....
Document: Performance of these four degenerate reverse transcription LAMP-OSD assays was optimized using singleplex reactions containing different amounts of synthetic target RNA derived from Asian lineage ZIKV (Supplementary Table T3 ). All four assays could detect a few hundred copies of synthetic target RNA within 60 min without producing spurious signal (Figure 2) . Furthermore, the high signal amplitude and low noise allowed simple yes/no visual readout and cellphone imaging of these assays -target RNA yielded bright visible fluorescence while assays lacking specific templates remained dark (Figure 2) .
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