Selected article for: "RNA synthesis and template dna"
Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus
  • Document date: 2018_9_23
    • ID: n5liudlg_40
    Snippet: Some 1000 ng of purified linear, double-stranded DNA transcription templates were transcribed using the HiScribe T7 High Yield RNA synthesis kit (NEB) according to the manufacturer's instructions. Transcription was allowed to occur at 37 °C for 2 h. Subsequently the transcription reactions were incubated with 2 units of DNase I (NEB) at 37 °C for 30 min to degrade the template DNA prior to RNA gel purification......
    Document: Some 1000 ng of purified linear, double-stranded DNA transcription templates were transcribed using the HiScribe T7 High Yield RNA synthesis kit (NEB) according to the manufacturer's instructions. Transcription was allowed to occur at 37 °C for 2 h. Subsequently the transcription reactions were incubated with 2 units of DNase I (NEB) at 37 °C for 30 min to degrade the template DNA prior to RNA gel purification.

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