Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus Document date: 2018_9_23
ID: n5liudlg_47
Snippet: LAMP-based assays intended for visual readout and smartphone imaging were assembled in 0.2 ml optically clear thin-walled tubes with low auto-fluorescence (Axygen, Union City, CA, USA). Following 90 -120 min of amplification at 65 °C, the reactions were imaged at room temperature using an unmodified iPhone 6 and an UltraSlim-LED transilluminator (Syngene, Frederick, MD, USA). In some experiments, our previously described in-house 3D-printed imag.....
Document: LAMP-based assays intended for visual readout and smartphone imaging were assembled in 0.2 ml optically clear thin-walled tubes with low auto-fluorescence (Axygen, Union City, CA, USA). Following 90 -120 min of amplification at 65 °C, the reactions were imaged at room temperature using an unmodified iPhone 6 and an UltraSlim-LED transilluminator (Syngene, Frederick, MD, USA). In some experiments, our previously described in-house 3D-printed imaging device 13 was used for fluorescence visualization and smartphone imaging. Briefly, this device uses Super Bright Blue 5 mm light emitting diodes (LED) (Adafruit, New York, NY, USA) to excite fluorescence. Two cut-to-fit layers of inexpensive >500 nm bandpass orange lighting gel sheets (Lee Filters, Burbank, CA, USA) on the observation window filter the fluorescence for observation and imaging.
Search related documents:
Co phrase search for related documents- auto fluorescence and smartphone imaging visual readout: 1
- auto fluorescence and USA Union City Axygen auto fluorescence: 1
- auto fluorescence and visual readout: 1
- gel sheet and room temperature: 1
- imaging device and smartphone imaging: 1
Co phrase search for related documents, hyperlinks ordered by date