Selected article for: "dual reporter and renilla luciferase"
Author: MJ Morwitzer; A Corona; L Zinzula; E Fanunza; C Nigri; S Distinto; C Vornholt; V Kumar; E Tramontano; SP Reid
Title: Mutation of Ebola virus VP35 Ser129 uncouples interferon antagonist and replication functions
  • Document date: 2019_8_7
    • ID: cbyzdnrp_73
    Snippet: Since VP35 is an essential polymerase cofactor, we next evaluated the effect of VP35-S129A 463 on EBOV minigenome activity. Plasmids encoding EBOV minigenome assay components NP, 464 L, VP35, VP30 and the 3E5E-luciferase minigenome, along with Renilla luciferase to control for 465 transfection efficiency variability, were co-transfected in HeLa cells. Forty-eight h post-466 transfection, a dual-luciferase assay was used to measure reporter activi.....
    Document: Since VP35 is an essential polymerase cofactor, we next evaluated the effect of VP35-S129A 463 on EBOV minigenome activity. Plasmids encoding EBOV minigenome assay components NP, 464 L, VP35, VP30 and the 3E5E-luciferase minigenome, along with Renilla luciferase to control for 465 transfection efficiency variability, were co-transfected in HeLa cells. Forty-eight h post-466 transfection, a dual-luciferase assay was used to measure reporter activity. Notably, the 467 presence of VP35-S129A nearly abolished minigenome activity relative to VP35-WT, with 468

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