Author: Ioanna Smyrlaki; Martin Ekman; Martin Vondracek; Natali Papanicoloau; Antonio Lentini; Johan Aarum; Shaman Muradrasoli; Jan Albert; Björn Högberg; Björn Reinius
Title: Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-qPCR Document date: 2020_4_17
ID: jeufhpkq_33
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is can be made by sample barcoding and high-throughput DNA sequencing. (f-g) Unlike the widely used approach, which includes an RNA extrac�on step (c) using industrial RNA extrac�on kits, direct sample tes�ng circumvents this process, by omi�ng extrac�on. Instead, a�er clinical samples are deposited in transport medium, viral par�cles are inac�vated either throug.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is can be made by sample barcoding and high-throughput DNA sequencing. (f-g) Unlike the widely used approach, which includes an RNA extrac�on step (c) using industrial RNA extrac�on kits, direct sample tes�ng circumvents this process, by omi�ng extrac�on. Instead, a�er clinical samples are deposited in transport medium, viral par�cles are inac�vated either through hea�ng or by direct lysis in detergent-containing buffer. The inac�vated samples are then used for the downstream RT-qPCR diagnos�c reac�on.
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