Author: MJ Morwitzer; A Corona; L Zinzula; E Fanunza; C Nigri; S Distinto; C Vornholt; V Kumar; E Tramontano; SP Reid
Title: Mutation of Ebola virus VP35 Ser129 uncouples interferon antagonist and replication functions Document date: 2019_8_7
ID: cbyzdnrp_47
Snippet: of FLAG-VP35-WT, demonstrating specificity of binding (Figure 2A, top panels) . Similarly, His-382 VP35-S129A was also pulled down in the presence of FLAG-VP35-WT, indicating that the 383 VP35-S129A mutant retains the ability to self-associate. Appropriate protein expression was 384 confirmed by WCL analysis (Figure 2A, bottom panels) . 385.....
Document: of FLAG-VP35-WT, demonstrating specificity of binding (Figure 2A, top panels) . Similarly, His-382 VP35-S129A was also pulled down in the presence of FLAG-VP35-WT, indicating that the 383 VP35-S129A mutant retains the ability to self-associate. Appropriate protein expression was 384 confirmed by WCL analysis (Figure 2A, bottom panels) . 385
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