A comprehensive annotation and differential expression analysis of short and long non-coding RNAs in 16 bat genomes

Nelly Mostajo Berrospi; Marie Lataretu; Sebastian Krautwurst; Florian Mock; Daniel Desirò; Kevin Lamkiewicz; Maximilian Collatz; Andreas Schoen; Friedemann Weber; Manja Marz; Martin Hölzer

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/738526 doi: bioRxiv preprint lncRNAs and lncRNA hot spots increases with increasing assembly quality (e.g. with a higher N50; see Fig. S1 ). However, this observation may be true for our data and analyses, but it also depends strongly on the annotation method used. Recently, the Bat1K project (http://bat1k.ucd.ie/) was announced as a global effort to sequence, assemble and annotate high-quality genomes of all living bat species 2 . We aim to extend our annotation of ncRNAs regulary and whenever new bat genomes become publicly available. In mid January 2019, 16 new low-coverage bat genomes of nine families were submitted by the Broad Institute to the NCBI genome database. Unfortunately, our time-consuming and computationally extensive analyses were already completed at this time point.