Selected article for: "generation sequencing and NGS generation sequencing"
Author: Felix Grünberger; Robert Knüppel; Michael Jüttner; Martin Fenk; Andreas Borst; Robert Reichelt; Winfried Hausner; Jörg Soppa; Sebastien Ferreira-Cerca; Dina Grohmann
Title: Nanopore-based native RNA sequencing provides insights into prokaryotic transcription, operon structures, rRNA maturation and modifications
  • Document date: 2019_12_19
    • ID: e5p4metz_1
    Snippet: In the last decade, next-generation sequencing (NGS) technologies 1 revolutionized the field of 54 microbiology 2 , which is not only reflected in the exponential increase in the number of fully 55 sequenced microbial genomes, but also in the detection of microbial diversity in many hitherto 56 inaccessible habitats based on metagenomics. Using transcriptomics, important advances were 57 also possible in the field of RNA biology 3,4 that shaped o.....
    Document: In the last decade, next-generation sequencing (NGS) technologies 1 revolutionized the field of 54 microbiology 2 , which is not only reflected in the exponential increase in the number of fully 55 sequenced microbial genomes, but also in the detection of microbial diversity in many hitherto 56 inaccessible habitats based on metagenomics. Using transcriptomics, important advances were 57 also possible in the field of RNA biology 3,4 that shaped our understanding of the transcriptional 58 landscape 5,6 and RNA-mediated regulatory processes in prokaryotes 7 . RNA sequencing (RNA-seq) 59 technologies can be categorized according to their platform-dependent read lengths and necessity 60 of a reverse transcription and amplification steps, to generate cDNA 8 . Illumina sequencing yields 61

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