Author: Nemudryi, Artem; Nemudraia, Anna; Wiegand, Tanner; Surya, Kevin; Buyukyoruk, Murat; Cicha, Calvin; Vanderwood, Karl K.; Wilkinson, Royce; Wiedenheft, Blake
Title: Temporal detection and phylogenetic assessment of SARS-CoV-2 in municipal wastewater Cord-id: khcxzeju Document date: 2020_8_31
ID: khcxzeju
Snippet: SARS-CoV-2 has recently been detected in feces, which indicates that wastewater may be used to monitor viral prevalence in the community. Here we use RT-qPCR to monitor wastewater for SARS-CoV-2 RNA over a 74-day time course. We show that changes in SARS-CoV-2 RNA concentrations follow symptom onset gathered by retrospective interview of patients but precedes clinical test results. Additionally, we determine a near complete (98.5%) SARS-CoV-2 genome sequence from the wastewater and use phylogene
Document: SARS-CoV-2 has recently been detected in feces, which indicates that wastewater may be used to monitor viral prevalence in the community. Here we use RT-qPCR to monitor wastewater for SARS-CoV-2 RNA over a 74-day time course. We show that changes in SARS-CoV-2 RNA concentrations follow symptom onset gathered by retrospective interview of patients but precedes clinical test results. Additionally, we determine a near complete (98.5%) SARS-CoV-2 genome sequence from the wastewater and use phylogenetic analysis to infer viral ancestry. Collectively, this work demonstrates how wastewater can be used as a proxy to monitor viral prevalence in the community and how genome sequencing can be used for genotyping viral strains circulating in a community.
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