Author: Deepak Kumar; Nitin Sharma; Murali Aarthy; Sanjeev Singh; Rajanish Giri
Title: Mechanistic insights into Zika virus NS3 helicase inhibition by Epigallocatechin-3-gallate: Supplementary Files Document date: 2019_1_26
ID: k11iupe0_19
Snippet: The Michaelis-Menten equation was used to quantitate the kinetic parameters (K m , K cat and K cat /K m ) for NTPase activity of NS3 helicase. The K m , K cat and K cat /K m were calculated as 345.9 ± 30.31 µM, 68.96 ± 1.71 min -1 and 0.1993 ± 0.056 µM -1 min -1 respectively ( Figure 6B ). Inhibition assays were carried out in triplicates and enzyme is preincubated for 10 minutes with varying concentrations of EGCG. Further, we have observed.....
Document: The Michaelis-Menten equation was used to quantitate the kinetic parameters (K m , K cat and K cat /K m ) for NTPase activity of NS3 helicase. The K m , K cat and K cat /K m were calculated as 345.9 ± 30.31 µM, 68.96 ± 1.71 min -1 and 0.1993 ± 0.056 µM -1 min -1 respectively ( Figure 6B ). Inhibition assays were carried out in triplicates and enzyme is preincubated for 10 minutes with varying concentrations of EGCG. Further, we have observed the dose dependent inhibition of NTPase activity and IC50 author/funder. All rights reserved. No reuse allowed without permission.
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