Molecular mechanisms of stress granule assembly and disassembly.

Hofmann, Sarah; Kedersha, Nancy; Anderson, Paul; Ivanov, Pavel

Stress granules (SGs) are membrane-less ribonucleoprotein-based cellular compartments that form in the cytoplasm of a cell upon exposure to various environmental stressors. SGs contain a large set of proteins, as well as mRNAs that have been stalled in translation as a result of stress-induced polysome disassembly. Despite the fact that SGs have been extensively studied for many years, their function is still not clear. They presumably help the cell to cope with the encountered stress, and facilitate the recovery process after stress removal, upon which SGs disassemble. Aberrant formation of SGs and impaired SG disassembly majorly contributes to various pathological phenomena in cancer, viral infections, and neurodegeneration. The assembly of SGs is largely driven by liquid-liquid phase separation (LLPS), however the molecular mechanisms behind that are not fully understood. Recent studies have proposed a novel mechanism for SG formation that involves the interplay of a large interaction network of mRNAs and proteins. Here we review this novel concept for the assembly of SGs, and discuss the current insights into SG disassembly.