Author: Charles L Howe; Reghann G. LaFrance-Corey; Emma N Goddery; Kanish Mirchia
Title: Neuronal CCL2 expression drives inflammatory monocyte infiltration into the brain during acute virus infection Document date: 2017_10_25
ID: ebqquj7i_5
Snippet: C57BL/6J (#000664), Syn-Cre (#003966), and CCL2-RFP fl/fl (#016849) mice were acquired from The Jackson Laboratories (Bar Harbor, ME). Mice were acclimatized for at least one week following shipment and prior to use or were bred in-house. Female mice between 4-6 weeks of age were used for all experiments. LysM:eGFP mice were maintained in-house, as described [4] . Syn-Cre and CCL2-RFP parents were bred in a our facility to establish double homozy.....
Document: C57BL/6J (#000664), Syn-Cre (#003966), and CCL2-RFP fl/fl (#016849) mice were acquired from The Jackson Laboratories (Bar Harbor, ME). Mice were acclimatized for at least one week following shipment and prior to use or were bred in-house. Female mice between 4-6 weeks of age were used for all experiments. LysM:eGFP mice were maintained in-house, as described [4] . Syn-Cre and CCL2-RFP parents were bred in a our facility to establish double homozygous F2 hybrids (Syn-Cre +/+ CCL2-RFP fl/fl ). All mice were group housed under controlled temperature and humidity with a 12 hour light/dark cycle with ad libitum access to food and water. All animal experiments were performed according to the National Institutes of Health guidelines and were approved by the Mayo Clinic Institutional Animal Care and Use Committee (Animal Welfare Assurance number A3291-01).
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