Multiple known and a novel parvovirus associated with an outbreak of feline diarrhea and vomiting

Yanpeng Li; Emilia Gordon; Amanda Idle; Eda Altan; M. Alexis Seguin; Marko Estrada; Xutao Deng; Eric Delwart

Cats have an estimated world-wide population of over half a billion. Members generate partial genome sequence. The genome gaps from the initial assembly were then filled 111 by PCR whose products were Sanger sequenced. 112 2.4. Diagnostic PCR and prevalence 113 DNA was extracted from each sample using the QIAamp MinElute Virus Spin Kit (Qiagen, 114 Hilden, Germany), and a semi-nested PCR assays was used for the detection of viral nucleic acids 115 in each sample. For fechavirus the first round PCR primers FechaF1 (5'-116 GGTGCGACGACGGAAGATAT-3') and FechaR1 (5'-CAACACCACCATCTCCTGCT-3') 117 Shelter 3 became involved in the outbreak when a cat (#160) transferred from Shelter 2 became 148 sick, several days after arrival and before the outbreak had been identified. Ultimately, a total 13 149 cats were affected in Shelter 1 in November, 17 cats were affected in Shelter 2 (November -150 January), and 13 cats were affected in Shelter 3 (November-January) (Figure 1 and 151 Supplementary Table 1 ). Nearly all transmission was indirect ( Figure 2) ; because of this, it was 152 not possible to definitively determine which animals had been exposed, except in specific rooms 153 where housing was communal or exposure was known to be widespread prior to introduction of 154 control measures. Attack rates for these rooms were 66.7% (Shelter 2) and 83.3% (Shelter 3) 155 (Table 1) . 156