Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus Document date: 2018_9_23
ID: n5liudlg_38
Snippet: For performing in vitro run-off transcription, ZIKV gene segments cloned in a pCR2.1-TOPO vector were amplified from sequenced plasmids by PCR using Phusion DNA polymerase. PCR products were verified by agarose gel electrophoresis and then purified using the Wizard SV gel and PCR Clean-up system (Promega, Madison, WI, USA), according to the manufacturer's instructions......
Document: For performing in vitro run-off transcription, ZIKV gene segments cloned in a pCR2.1-TOPO vector were amplified from sequenced plasmids by PCR using Phusion DNA polymerase. PCR products were verified by agarose gel electrophoresis and then purified using the Wizard SV gel and PCR Clean-up system (Promega, Madison, WI, USA), according to the manufacturer's instructions.
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