Author: William Fitzsimmons; Robert J. Woods; John T. McCrone; Andrew Woodman; Jamie J. Arnold; Madhumita Yennawar; Richard Evans; Craig E. Cameron; Adam S. Lauring
Title: A speed-fidelity trade-off determines the mutation rate and virulence of an RNA virus Document date: 2018_4_27
ID: 8p24gszj_67
Snippet: After two days the media were passaged onto a separate monolayer of HeLa cells. Upon 455 cytopathic effect, viruses were harvested by three repeated freeze-thaw cycles, cell debris was 456 removed by centrifugation, and viral supernatants were titrated. In this time frame, the titer 457 increased approximately 40-fold (from 5.1 x 10 5 pfu/mL to 2.1 x 10 7 ). Viral RNA was isolated 458 with QIAamp viral RNA purification kits (Qiagen), according to.....
Document: After two days the media were passaged onto a separate monolayer of HeLa cells. Upon 455 cytopathic effect, viruses were harvested by three repeated freeze-thaw cycles, cell debris was 456 removed by centrifugation, and viral supernatants were titrated. In this time frame, the titer 457 increased approximately 40-fold (from 5.1 x 10 5 pfu/mL to 2.1 x 10 7 ). Viral RNA was isolated 458 with QIAamp viral RNA purification kits (Qiagen), according to the manufacturer's instructions.
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