Author: Ria Lassaunière; Anders Frische; Zitta B Harboe; Alex CY Nielsen; Anders Fomsgaard; Karen A Krogfelt; Charlotte S Jørgensen
Title: Evaluation of nine commercial SARS-CoV-2 immunoassays Document date: 2020_4_10
ID: 8cg5yj20_4
Snippet: Following 30 minutes of incubation at room temperature, the reaction was stopped and the resultant absorbance was read on a microplate reader at 450 nm with reference at 620 nm. A ratio between the extinction of the sample and calibrator on each plate were calculated. According to the manufacturer's recommendations, a ratio <0.8 is considered negative, ≥0.8 and <1.1 borderline, and ≥1.1 positive. However, for sensitivity and specificity, 1.1 .....
Document: Following 30 minutes of incubation at room temperature, the reaction was stopped and the resultant absorbance was read on a microplate reader at 450 nm with reference at 620 nm. A ratio between the extinction of the sample and calibrator on each plate were calculated. According to the manufacturer's recommendations, a ratio <0.8 is considered negative, ≥0.8 and <1.1 borderline, and ≥1.1 positive. However, for sensitivity and specificity, 1.1 was used as a more stringent cut-off value for positive results and all values <1.1 were considered negative.
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