Author: Pingping Zhang; Qi Gao; Tang Wang; Yuehua Ke; Fei Mo; Ruizhong Jia; Wanbing Liu; Lei Liu; Shangen Zheng; Yuzhen Liu; Luping Li; Yao Wang; Lei Xu; Kun Hao; Ruifu Yang; Shiyue Li; Changqing Lin; Yong Zhao
Title: Evaluation of recombinant nucleocapsid and spike proteins for serological diagnosis of novel coronavirus disease 2019 (COVID-19) Document date: 2020_3_20
ID: 7atoko6c_18
Snippet: BioDot, Irvine, CA), rS1 and the secondary polyclonal antibody (2 mg/mL) were 5 coated onto the nitrocellulose membrane as the test and control lines, respectively, at a 6 dispensing rate of 1.0 μ L/cm. The membrane was then dried at 37 °C for 1 h. Finally, 7 the nitrocellulose membrane, conjugate pad, sample pad and the absorbent pads were 8 assembled and cut into 4-mm strips. 9.....
Document: BioDot, Irvine, CA), rS1 and the secondary polyclonal antibody (2 mg/mL) were 5 coated onto the nitrocellulose membrane as the test and control lines, respectively, at a 6 dispensing rate of 1.0 μ L/cm. The membrane was then dried at 37 °C for 1 h. Finally, 7 the nitrocellulose membrane, conjugate pad, sample pad and the absorbent pads were 8 assembled and cut into 4-mm strips. 9
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