Author: Ali Punjani; Haowei Zhang; David J. Fleet
Title: Non-uniform refinement: Adaptive regularization improves single particle cryo-EM reconstruction Document date: 2019_12_16
ID: bqwmx5dy_38
Snippet: In the original STRA6-CaM publication using amphipol, the dataset of 56,615 particles was refined to 3.9Ã… using a uniform refinement. In the result presented here, about half the number of particles (28, 848) in lipid nanodisc yield a uniform refinement result of 4.0Ã… indicating that the quality of the data is higher. Non-uniform refinement, however, produces a 3.6Ã… result that is substantially better than both uniform refinements......
Document: In the original STRA6-CaM publication using amphipol, the dataset of 56,615 particles was refined to 3.9Ã… using a uniform refinement. In the result presented here, about half the number of particles (28, 848) in lipid nanodisc yield a uniform refinement result of 4.0Ã… indicating that the quality of the data is higher. Non-uniform refinement, however, produces a 3.6Ã… result that is substantially better than both uniform refinements.
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