Author: Craig Thompson; Nicholas Grayson; Robert Paton; José Lourenço; Bridget Penman; Lian Ni Lee; Valerie Odon; Juthathip Mongkolsapaya; Senthil Chinnakannan; Wanwisa Dejnirattisai; Matthew Edmans; Alexander Fyfe; Carol Imlach; Kreepa Kooblall; Nicholas Lim; Chang Liu; Cesar Lopez-Camacho; Carol-Anne McInally; Narayan Ramamurthy; Jeremy Ratcliff; Piyada Supasa; Beibei Wang; Alexander J Mentzer; Marc Turner; Calum Semple; John Kenneth Baillie; Heli Harvala; Gavin Screaton; Nigel Temperton; Paul Klenerman; Lisa Jarvis; Sunetra Gupta; Peter Simmonds
Title: Neutralising antibodies to SARS coronavirus 2 in Scottish blood donors - a pilot study of the value of serology to determine population exposure Document date: 2020_4_17
ID: nm2bq717_8
Snippet: StrepMAB-Classic (2-1507-001;iba). Plates were blocked with 2% skimmed milk in PBS for one hour and then incubated with 0.125ug of soluble trimeric SARS-CoV2 trimeric Spike protein or 2% skimmed milk in PBS. After one hour, plasma was added at 1:50 dilution, followed by ALP-conjugated anti-human IgG (A9544; Sigma) at 1:10,000 dilution or ALPconjugated anti-human IgM (A9794; Sigma) at 1:5,000 dilution. The reaction was developed 150 by the additio.....
Document: StrepMAB-Classic (2-1507-001;iba). Plates were blocked with 2% skimmed milk in PBS for one hour and then incubated with 0.125ug of soluble trimeric SARS-CoV2 trimeric Spike protein or 2% skimmed milk in PBS. After one hour, plasma was added at 1:50 dilution, followed by ALP-conjugated anti-human IgG (A9544; Sigma) at 1:10,000 dilution or ALPconjugated anti-human IgM (A9794; Sigma) at 1:5,000 dilution. The reaction was developed 150 by the addition of PNPP substrate and stopped with NaOH. The absorbance was measured at 405nm after 1 hour.
Search related documents:
Co phrase search for related documents- milk skim and Spike protein: 1
- PBS milk skim and Spike protein: 1
- Spike protein and trimeric Spike protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
Co phrase search for related documents, hyperlinks ordered by date