Author: Rui Xiong; Leike Zhang; Shiliang Li; Yuan Sun; Minyi Ding; Yong Wang; Yongliang Zhao; Yan Wu; Weijuan Shang; Xiaming Jiang; Jiwei Shan; Zihao Shen; Yi Tong; Liuxin Xu; Chen Yu; Yingle Liu; Gang Zou; Dimitri Lavillete; Zhenjiang Zhao; Rui Wang; Lili Zhu; Gengfu Xiao; Ke Lan; Honglin Li; Ke Xu
Title: Novel and potent inhibitors targeting DHODH, a rate-limiting enzyme in de novo pyrimidine biosynthesis, are broad-spectrum antiviral against RNA viruses including newly emerged coronavirus SARS-CoV-2 Document date: 2020_3_12
ID: hq5um68k_24
Snippet: . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . (Supplementary Fig. 3 ). By contrast, virus growth was largely inhibited in DHODH -/cells as compared to wild-type (WT) A549 cells with almost 1000-fold reduction of infectious particles at 72 hours post-infection (h.p.i.) (Fig. 4B) . When S312 (5IC50) was added into the culture medium, .....
Document: . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . (Supplementary Fig. 3 ). By contrast, virus growth was largely inhibited in DHODH -/cells as compared to wild-type (WT) A549 cells with almost 1000-fold reduction of infectious particles at 72 hours post-infection (h.p.i.) (Fig. 4B) . When S312 (5IC50) was added into the culture medium, dramatic reduction of virus growth only occurred in WT cells but not in DHODH -/cells ( Fig. 4C and 4D) . These results prove that virus growth but not the coincident cell growth requires DHODH activity, and antiviral action of S312 is implemented by targeting DHODH.
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