Author: Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek
Title: Messenger RNAs transcribed from yeast linear cytoplasmic plasmids possess unconventional 5’ and 3’ UTRs and suggest a novel mechanism of translation Document date: 2018_5_17
ID: foskvkwn_7
Snippet: In this work, we focus on the yet uncharacterized transcriptome of the yeast cytoplasmic linear dsDNA plasmids and the possible mechanism of translation initiation used by the plasmid-specific mRNAs. We demonstrate that pGKL mRNAs are not 3' polyadenylated and that most of them even lack the m 7 G cap at their 5' ends. Surprisingly, mRNAs transcribed from pGKL plasmids contain poly(A) leaders of variable lengths at their 5' ends that are not comp.....
Document: In this work, we focus on the yet uncharacterized transcriptome of the yeast cytoplasmic linear dsDNA plasmids and the possible mechanism of translation initiation used by the plasmid-specific mRNAs. We demonstrate that pGKL mRNAs are not 3' polyadenylated and that most of them even lack the m 7 G cap at their 5' ends. Surprisingly, mRNAs transcribed from pGKL plasmids contain poly(A) leaders of variable lengths at their 5' ends that are not complementary to the plasmid DNA sequence and must thus be Total yeast RNA was purified by the hot acidic phenol procedure (40). Remaining DNA was removed by a DNA-free Kit (Ambion) according to the manufacturer's protocol.
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