Author: David Brann; Tatsuya Tsukahara; Caleb Weinreb; Darren W. Logan; Sandeep Robert Datta
Title: Non-neural expression of SARS-CoV-2 entry genes in the olfactory epithelium suggests mechanisms underlying anosmia in COVID-19 patients Document date: 2020_3_27
ID: bb4h255w_50
Snippet: Human olfactory mucosa biopsies were obtained via IRB-approved protocol at Duke University School of Medicine, from nasal septum or superior turbinate during endoscopic sinus surgery. Tissue was fixed with 4% paraformaldehyde and cryosectioned at 10 ïm and sections were processed for immunohistochemistry, as previously described (44) . Primary antibodies were applied overnight at 4 degrees. Primary antibodies included rabbit anti-ACE2 (Abcam, a.....
Document: Human olfactory mucosa biopsies were obtained via IRB-approved protocol at Duke University School of Medicine, from nasal septum or superior turbinate during endoscopic sinus surgery. Tissue was fixed with 4% paraformaldehyde and cryosectioned at 10 ïm and sections were processed for immunohistochemistry, as previously described (44) . Primary antibodies were applied overnight at 4 degrees. Primary antibodies included rabbit anti-ACE2 (Abcam, ab15348; RRID:AB_301861) and mouse Tuj1 against neuron-specific tubulin (BioLegend, 801201; RRID:AB_2313773). Biotinylated secondary antibodies (Vector Labs), avidin-biotinylated horseradish peroxidase kit (Vector) followed by fluorescein tyramide signal amplification (Perkin Elmer) were applied per manufacturer's instructions. For dual staining, Tuj1 was author/funder. All rights reserved. No reuse allowed without permission.
Search related documents:
Co phrase search for related documents- human olfactory mucosa biopsy and mucosa biopsy: 1
Co phrase search for related documents, hyperlinks ordered by date