Author: C Langelier; MS Zinter; K Kalantar; GA Yanik; S Christenson; B Odonovan; C White; M Wilson; A Sapru; CC Dvorak; S Miller; CY Chiu; JL DeRisi
Title: Metagenomic Sequencing Detects Respiratory Pathogens in Hematopoietic Cellular Transplant Patients Document date: 2017_1_24
ID: km0b1z64_13
Snippet: To mitigate spurious taxonomic assignments due to laboratory background contaminants, we calculated a standard Z-score for each microbial genus relative to a control group of 17 "no-template" water-only controls (detailed in Table E1 ) derived from sequencing runs performed in our laboratory over the past 2 years (24). Z-scores were calculated based on unique reads aligned to either NCBI nt or nr, per million sequence input reads (rpM nt , rpM nr.....
Document: To mitigate spurious taxonomic assignments due to laboratory background contaminants, we calculated a standard Z-score for each microbial genus relative to a control group of 17 "no-template" water-only controls (detailed in Table E1 ) derived from sequencing runs performed in our laboratory over the past 2 years (24). Z-scores were calculated based on unique reads aligned to either NCBI nt or nr, per million sequence input reads (rpM nt , rpM nr ).
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