Selected article for: "16S rrna gene pcr and rrna gene"

Author: C Langelier; MS Zinter; K Kalantar; GA Yanik; S Christenson; B Odonovan; C White; M Wilson; A Sapru; CC Dvorak; S Miller; CY Chiu; JL DeRisi
Title: Metagenomic Sequencing Detects Respiratory Pathogens in Hematopoietic Cellular Transplant Patients
  • Document date: 2017_1_24
  • ID: km0b1z64_17
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/102798 doi: bioRxiv preprint Page 10 of 29 potential pathogens were confirmed by independent specific PCR testing for viruses and 16s rRNA gene Sanger sequencing for bacteria. Finally, microbes were considered unlikely or uncertain pathogens if they a) lacked robust literature evidence of microbial pathogenicity in the lungs, b) ha.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/102798 doi: bioRxiv preprint Page 10 of 29 potential pathogens were confirmed by independent specific PCR testing for viruses and 16s rRNA gene Sanger sequencing for bacteria. Finally, microbes were considered unlikely or uncertain pathogens if they a) lacked robust literature evidence of microbial pathogenicity in the lungs, b) had a score that was less than two-fold that of the top-ranking microbe, suggesting a polymicrobial sample, c) determined to be clinically insignificant by the treatment team, or d) were a DNA virus of uncertain pathogenicity present in low abundance (< 5rpM).

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