Selected article for: "dna extraction and spin kit"

Author: Aran Singanayagam; Joseph Footitt; Benjamin T Kasdorf; Matthias Marczynski; Michael T Cross; Lydia J Finney; Maria-Belen Trujillo Torralbo; Maria Calderazzo; Jie Zhu; Julia Aniscenko; Thomas B Clarke; Philip L Molyneaux; Nathan W Bartlett; Miriam F Moffatt; William O Cookson; Jadwiga Wedzicha; Christopher M Evans; Oliver Lieleg; Patrick Mallia; Sebastian L Johnston
Title: MUC5AC drives COPD exacerbation severity through amplification of virus-induced airway inflammation
  • Document date: 2019_7_22
  • ID: gg2ctmn7_72
    Snippet: DNA extraction from human sputum and mouse lung was performed using the FastDNA Spin Kit for Soil (MP Biomedicals, USA), following manufacturer instructions. Bead-beating was performed for two cycles of 30 seconds at 6800 rpm (Precellys, Bertin Technologies, France). 16S bacterial loads were measured using qPCR, as previously described 50 ......
    Document: DNA extraction from human sputum and mouse lung was performed using the FastDNA Spin Kit for Soil (MP Biomedicals, USA), following manufacturer instructions. Bead-beating was performed for two cycles of 30 seconds at 6800 rpm (Precellys, Bertin Technologies, France). 16S bacterial loads were measured using qPCR, as previously described 50 .

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