Author: Aran Singanayagam; Joseph Footitt; Benjamin T Kasdorf; Matthias Marczynski; Michael T Cross; Lydia J Finney; Maria-Belen Trujillo Torralbo; Maria Calderazzo; Jie Zhu; Julia Aniscenko; Thomas B Clarke; Philip L Molyneaux; Nathan W Bartlett; Miriam F Moffatt; William O Cookson; Jadwiga Wedzicha; Christopher M Evans; Oliver Lieleg; Patrick Mallia; Sebastian L Johnston
Title: MUC5AC drives COPD exacerbation severity through amplification of virus-induced airway inflammation Document date: 2019_7_22
ID: gg2ctmn7_85
Snippet: Lyophilized bovine salivary mucin MUC5B powder was purchased from Merck (499643, Darmstadt, Germany) and dissolved in 10 mM sodium phosphate buffer (pH 7.0, supplemented with 1 M NaCl) at a concentration of 1 mg mL -1 and the solution was rotated on a rotating incubator for 1 h at 4 °C. Further purification of salivary mucin MUC5B was conducted according to the protocol used for purifying gastric mucin MUC5AC as described above......
Document: Lyophilized bovine salivary mucin MUC5B powder was purchased from Merck (499643, Darmstadt, Germany) and dissolved in 10 mM sodium phosphate buffer (pH 7.0, supplemented with 1 M NaCl) at a concentration of 1 mg mL -1 and the solution was rotated on a rotating incubator for 1 h at 4 °C. Further purification of salivary mucin MUC5B was conducted according to the protocol used for purifying gastric mucin MUC5AC as described above.
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