Author: Alexander M. Price; Katharina E. Hayer; Daniel P. Depledge; Angus C. Wilson; Matthew D. Weitzman
Title: Novel splicing and open reading frames revealed by long-read direct RNA sequencing of adenovirus transcripts Document date: 2019_12_13
ID: 2fcl6f4i_8
Snippet: antagonizing intrinsic cellular defenses [31] [32] [33] the quality and depth of current short-read sequencing technologies is high, the complex nature 117 of many viral transcriptomes precludes the unambiguous mapping of these short reads to any 118 one particular RNA isoform due to extreme gene density and overlapping transcriptional units 119 [55, 56] . In this regard, the ability of long-read RNA sequencing to map full-length transcripts has .....
Document: antagonizing intrinsic cellular defenses [31] [32] [33] the quality and depth of current short-read sequencing technologies is high, the complex nature 117 of many viral transcriptomes precludes the unambiguous mapping of these short reads to any 118 one particular RNA isoform due to extreme gene density and overlapping transcriptional units 119 [55, 56] . In this regard, the ability of long-read RNA sequencing to map full-length transcripts has 120 the potential to revolutionize detection of divergent isoforms and multiply spliced RNA at the 121 single-molecule level [57] [58] [59] . 122
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