Selected article for: "extraction step and high throughput"
Author: Alcoba-Florez, Julia; Gonzalez-Montelongo, Rafaela; Inigo-Campos, Antonio; Garcia-Martinez de Artola, Diego; Gil-Campesino, Helena; Ciuffreda, Laura; Valenzuela-Fernandez, Agustin; Flores, Carlos
Title: Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples
  • Cord-id: icpwfyss
  • Document date: 2020_4_11
    • ID: icpwfyss
    Snippet: The current reference for COVID-19 diagnosis is based on the detection of SARS-CoV-2 on RNA extracts using one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput COVID-19 screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, overcoming the long and tedious RNA extraction step. Although with an average increase of 6.1 ( ±1.6) cycles compared to standard tests with RNA extracts, we
    Document: The current reference for COVID-19 diagnosis is based on the detection of SARS-CoV-2 on RNA extracts using one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput COVID-19 screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, overcoming the long and tedious RNA extraction step. Although with an average increase of 6.1 ( ±1.6) cycles compared to standard tests with RNA extracts, we show that RT-qPCR yielded consistent results in nasopharyngeal swab samples that were subject to a direct 70°C incubation for 10 min. Our findings provide viable options to overcome any supply chain issue and help to increase the throughput of diagnostic tests by using any qPCR device, thereby complementing standard COVID-19 testing.

    Search related documents:
    Co phrase search for related documents
    • accuracy specificity and acid extraction: 1, 2
    • accuracy specificity and actb gene amplification: 1
    • accuracy specificity and acute respiratory: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • accuracy specificity sensitivity and actb gene amplification: 1
    • accuracy specificity sensitivity and acute respiratory: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • acid extraction and actb gene expression: 1
    • acid extraction and acute respiratory: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • acid extraction and magcore liquid handler: 1
    • actb gene expression and magcore liquid handler: 1