Author: Myra Hosmillo; Jia Lu; Michael R. McAllaster; James B. Eaglesham; Xinjie Wang; Edward Emmott; Patricia Domingues; Yasmin Chaudhry; Timothy J Fitzmaurice; Matthew K.H. Tung; Marc Panas; Gerald McInerney; Nicholas Locker; Craig B. Willen; Ian Goodfellow
Title: Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation Document date: 2019_3_8
ID: d0q5lhf4_14
Snippet: Transfection of replicon RNA, purified from replicon containing cells, into BHK cells 217 readily resulted in the formation of antibiotic resistant cell colonies ( Fig 5A) . In 218 contrast, RNA that was proteinase K treated prior to transfection was unable to 219 produce replicon containing colonies. Transfection of replicon RNA into wild type 220 U2OS osteosarcoma cells allowed the formation of replicon-containing colonies, 221 although the eff.....
Document: Transfection of replicon RNA, purified from replicon containing cells, into BHK cells 217 readily resulted in the formation of antibiotic resistant cell colonies ( Fig 5A) . In 218 contrast, RNA that was proteinase K treated prior to transfection was unable to 219 produce replicon containing colonies. Transfection of replicon RNA into wild type 220 U2OS osteosarcoma cells allowed the formation of replicon-containing colonies, 221 although the efficiency of formation was significantly less than that seen in BHK cells 222 ( Fig 5B) . CRISPR modified U2OS cells that lacked G3BP1 (Kedersha et al., 2016) 223 were unable to support NV replication, as evident by the lack of antibiotic resistant 224 colonies ( Fig 5B) . To further examine the role of G3BP1 in human Norwalk virus 225 replication, WT or G3BP1 deficient U2OS cells were transfected with NV replicon 226
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