The expression of HSPs, anti-oxidants, and cytokines in plasma and bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome

Lee, Eun Joo; Lim, Ji-Youn; Lee, Sang Yeub; Lee, Seung Hyeon; In, Kwang Ho; Yoo, Se Hwa; Sul, Donggeun; Park, Sanghoon

OBJECTIVES: We studied several acute inflammatory materials (AIM) such as various inflammatory cytokines, oxidative stress, and heat shock proteins in ARDS patients by simultaneously measuring from bronchoalveolar lavage fluid (BALF) and plasma. DESIGN AND METHODS: AIM were measured by using plasma and BALF sampling obtained from ARDS group (n = 12) and non-ARDS group (n = 12). RESULTS: In the BALF, only HSP 47 was significantly increased in ARDS group than non-ARDS group. In plasma, GRP 94, HSP 90, HSP 60, HSP 47, GPx-3, and IL-8 were increased significantly in ARDS group. In short, most of the AIM in BALF or plasma were not significantly different in ARDS group as compared with non-ARDS group. Ninety-day mortality was significantly related to HSP90, HSP 60 and GPx-3 in plasma but not in BALF. CONCLUSION: Alteration of AIM levels in both BALF or plasma of ARDS group was not remarkable compared with the non-ARDS group. Our result suggests the need to reconsider ARDS pathophysiology and therapeutic strategy.