Author: Megan C. Cohan; Ammon E. Posey; Steven J. Grigsby; Anuradha Mittal; Alex S. Holehouse; Paul J. Buske; Petra A. Levin; Rohit V. Pappu
Title: Evolved sequence features within the intrinsically disordered tail influence FtsZ assembly and bacterial cell division Document date: 2018_4_14
ID: 2rzfuy33_93
Snippet: Assembly reactions contained 5 µM FtsZ in an MES buffer solution (50 mM MES, 2.5 mM MgCl2, 1 mM EGTA, 10% sucrose, pH 6.5, with salt concentrations varying from 50-200 mM KCl as specified). Measurements were recorded every quarter of a second at 30°C. A 1-minute baseline was established prior to adding 1 mM GTP to the reaction. A minimum of three trials was conducted per salt concentration for each variant. All data were collected and exported .....
Document: Assembly reactions contained 5 µM FtsZ in an MES buffer solution (50 mM MES, 2.5 mM MgCl2, 1 mM EGTA, 10% sucrose, pH 6.5, with salt concentrations varying from 50-200 mM KCl as specified). Measurements were recorded every quarter of a second at 30°C. A 1-minute baseline was established prior to adding 1 mM GTP to the reaction. A minimum of three trials was conducted per salt concentration for each variant. All data were collected and exported into Microsoft Excel, and the subsequent analysis was performed using Matlab. The average baseline was subtracted from each data point.
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