Author: Megan C. Cohan; Ammon E. Posey; Steven J. Grigsby; Anuradha Mittal; Alex S. Holehouse; Paul J. Buske; Petra A. Levin; Rohit V. Pappu
Title: Evolved sequence features within the intrinsically disordered tail influence FtsZ assembly and bacterial cell division Document date: 2018_4_14
ID: 2rzfuy33_95
Snippet: Samples were prepared in conditions mimicking the light scattering assays, with a lower concentration of FtsZ (2.5 µM). Prior to preparing the copper grids, each sample was incubated for 10 minutes in the presence of 1 mM GTP to allow for adequate assembly. Each sample was stained 3x with 2% uranyl acetate for 20-seconds each, with the solution wicked away and followed by a 10 second wait time in between stains. Samples were visualized using a F.....
Document: Samples were prepared in conditions mimicking the light scattering assays, with a lower concentration of FtsZ (2.5 µM). Prior to preparing the copper grids, each sample was incubated for 10 minutes in the presence of 1 mM GTP to allow for adequate assembly. Each sample was stained 3x with 2% uranyl acetate for 20-seconds each, with the solution wicked away and followed by a 10 second wait time in between stains. Samples were visualized using a FEI Transmission Electron Microscope.
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