Selected article for: "chain reaction and nucleic acid detection"
Author: Prabhakar, Pranav Kumar; Lakhanpal, Jyoti
Title: Recent advances in the nucleic acid-based diagnostic tool for coronavirus
  • Cord-id: jy8euh6b
  • Document date: 2020_10_6
    • ID: jy8euh6b
    Snippet: ABSTRACT: Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but a
    Document: ABSTRACT: Recently in China, a novel coronavirus outbreak took place which caused pneumonia-like symptoms. This coronavirus belongs to the family of SARS and MERS and causes respiratory system disease known as COVID-19. At present we use polymerase chain reaction (PCR) based molecular biology methods for the detection of coronavirus. Other than these PCR based methods, some improved methods also exist such as microarray-based techniques, Real time-quantitative PCR, CRISPR-Cas13 based tools but almost all of the available methods have advantages and disadvantages. There are many limitations associated with this method and hence there is a need for a fast, more sensitive, and specific diagnostic tool which can detect a greater number of samples in less time. Here we have summarised currently available nucleic acid-based diagnostic methods for the detection of coronavirus and the need for developing a better technique for a fast and sensitive detection of coronavirus infections. GRAPHIC ABSTRACT: Nucleic acid based detection tool for SARS-CoV-2. [Image: see text]

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